Updated on Feb. 28, 2008

BIBLIOGRAPHY
Wolf-Dietrich Heyer

2008: Ehmsen, K. and Heyer, W.-D. (2008) Biochemistry of Meiotic Recombination: Formation, Processing, and Resolution of Recombination Intermediates Genome Dynamics & Stability, 1861-3373, 2008. [PDF] Ehmsen, K. and Heyer, W.-D. (2008) Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease Nucleic Acids Research, Advance Access 10.1093/nar/gkm1152. [PubMed][PDF] This paper has been selected for Faculty of 1000 Biology. See the Evaluations. Li, X. and Heyer, W.-D. (2008) Homologous recombination in DNA repair and DNA damage tolerance. Cell Res. 18 (1):99-113. [PubMed][PDF]

2007: Heyer, W.-D. (2007) Biochemistry of eukaryotic homologous recombination. In Topics in Current Genetics. vol 17: Molecular Genetics of Recombination, Pp 95-133. [PDF] Li, X., Zhang, X.-P., Solinger, J., Kiianitsa, K., Yu, X., Egelman, E. and Heyer, W.-D. (2007) Rad51 and Rad54 ATPase activites are both required to modulate Rad51-dsDNA filament dynamics. Nucleic Acid Res. 35(12): 4124-4140. [PubMed] [PDF]. Mimida, N., Kitamoto, H., Osakabe, K., Nakashima, M., Ito, Y. Heyer, W.-D., Toki, S. and Ichikawa, H.  (2007) Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice Homolog of Yeast MUS81, Are Up-Regulated by DNA-Damaging Treatments Plant Cell Physiol, 48 (4): 648-54 [PubMed] [PDF]

2006: Symington, L. and Heyer, W.-D. (2006) Some disassembly required: role of DNA translocases in the disruption of recombination intermediates and dead-end complexes. Genes Dev. 20(18): 2479-2486. [PubMed] [PDF] Herzberg, K, Bashkirov, V.I., Rolfsmeier, M., Haghnazari. E, McDonald, W.H., Anderson, S, Bashkirova, E.V., Yates, J.R. 3rd. and Heyer, W.-D.(2006) Phosphorylation of Rad55 on serines 2, 8, and 14 is required for efficient homologous recombination in the recovery of stalled replication forks. Mol. Cell Biol. 26(22): 8396-8409. [PubMed] [PDF] Chin, J.K., Bashkirov, V.I., Heyer, W.-D. and Romesberg, F.E. (2006) Esc4/Rtt107 and the control of recombination during replication. DNA Repair (Amst). 5(5): 618-628.[PubMed] [PDF] Kiianitsa, K., Solinger, J.A. and W.-D. Heyer. (2006) Rad54 protein disassembles Rad51-dsDNA complexes by terminal association with the nucleoprotein filament. PNAS, 103(26): 9767-9772. [PubMed] [PDF] Galkin, V.E., Wu, Y., Zhang, X.-P., Qian, X., He, Y., Yu, X., Heyer, E.-D., Luo, Y. and Egelman, E.H. (2006) The Rad51/RadA N-terminal domain activates Nucleoprotein Filaments. STRUCTURE, 14, 983-992. [PubMed] [PDF] [Cover Illustration] [Preview by Wyman, C. (2006) Monomer Networking Activates Recombinases. Structure 14(6): 949-950 [PDF] ] Heyer, W.-D., Li, X., Rolfsmeier, M. and Zhang, X.-P. (2006) Rad54, the Swiss Army knife of homologous recombination? Nucleic Acid Res, 34(15): 4115-4125 .[PubMed] [PDF]   Bashkirov, V.I., Herzberg, K., Haghnazari, E., Vlasenko, A. and Heyer, W.-D. (2006) DNA damage-induced phosphorylation of Rad55 protein as a sentinel for DNA damage checkpoint activation in S. cerevisiae. Meth. Enzymol. 409:166-182. [Pub Med] [PDF]

2005: Zhang, X.-P., Lee, K.-I., Solinger, J.A., Kiianitsa, K., and Heyer, W.-D. (2005) Gly103 in the N-terminal domain of S. cerevisiae Rad51 protein is critical for DNA binding. J. Biol. Chem. 280, 26303-26311. [PubMed] [PDF]

2004: Heyer, W.-D. and Kanaar, R. (2004) Recombination Mechanisms; fortieth anniversary meeting of the Holliday model. Mol. Cell 16, 1-20.[PubMed][PDF] Heyer, W.-D. (2004) Damage signaling: RecQ sends an SOS to you. Curr. Biol. 14, R895-R897. [PubMed] [PDF] Haghnazari, E. and Heyer, W.-D. (2004a) The Hog1 MAP kinase pathway and the Mec1 DNA damage checkpoint pathway independently control the cellular responses to hydrogen peroxide. DNA Repair 3, 769-776. [PubMed] [PDF] Haghnazari, E. and Heyer, W.-D. (2004b) The DNA damage checkpoint pathways exert multiple controls on the efficiency and outcome of the repair of a double stranded DNA gap. Nucleic Acids Res. 32, 4257-4268. [PubMed] [PDF]   Heyer, W.-D. (2004) Recombination: Mus81 in Holliday junction resolution and crossover formation. Curr. Biol. 14, R56-R58. [PubMed] [PDF] Heyer, W.-D. (2004) A new deal for Holliday junctions. Nature Struct. Mol. Biol. 11, 117-119. [Abstract] [PDF Local]

2003: Bashkirov, V.I., Bashkirov, E.V., Haghnazari, E. and Heyer, W.-D. (2003) Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Mol. Cell. Biol. 23, 1441-1452. [PubMed] [PDF] Schmuckli-Maurer, J., Rolfsmeier, M., Nguyen, H. and Heyer, W.-D. (2003) Genome instability in RAD54 mutants of Saccharomyces cerevisiae. Nucleic Acids Res. 31, 1013-1023. [PubMed] [PDF]   Mallory, J.C., Bashkirov, V.I., Trujillo, K.M., Solinger, J.A., Dominska, M., Sung, P., Heyer, W.D. and Petes, T.D. (2003) Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair 2, 1041-1064. [PubMed] [PDF]   Kiianitsa, K., Solinger, J. and Heyer, W.-D. (2003) NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities. Anal. Biochem. 321, 266-271. [PubMed] [PDF]   Heyer, W.-D., Ehmsen, K.T. and Solinger, J.A. (2003) Holliday junctions: Resolution in sight? TiBS 10, 548-557.[PubMed] [PDF]

2002: Fabre, F., Chan, A., Heyer, W.-D. and Gangloff, S.(2002) Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication. Proc Natl Acad Sci USA. 99,16887-16892. [PubMed] [PDF Local] Solinger, J.A., Kiianitsa, K., and Heyer, W.-D. (2002) Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments. Mol Cell.10, 1175-1188. [PubMed] [PDF] Kiianitsa, K., Solinger, J.A. and Heyer, W.-D. (2002) Rad54 protein exerts diverse modes of ATPase activity on duplex DNA partially and fully covered with Rad51 protein. J Biol Chem. 277, 46205-46215. [[PubMed][PDF] Walsh, L., Schmuckli-Maurer, J., Billinton, N., Barker, M.G., Heyer, W.-D., and Walmsley, R.M. (2002) DNA-damage induction of RAD54 can be regulated independently of the RAD9- and DDC1-dependent checkpoints that regulate RNR2. Curr Genet. 41, 232-240. [PubMed] [PDF] Kim, P.M., Paffett, K.S., Solinger, J.A., Heyer, W.-D. and Nickoloff, J.A. (2002) Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54. Nucleic Acids Res. 30, 2727-2735. [PubMed] [PDF]

2001: Solinger, J.A. and Heyer, W.-D. (2001) Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange. Proc. Natl. Acad. Sci. USA. 98, 8447-8453. [PubMed] [PDF] Solinger, J.A., Lutz, G., Sugiyama, T., Kowalczykowski, S.C., and Heyer, W.-D. (2001) Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament. J. Mol. Biol. 307, 1207-1221. [PubMed] [PDF] Heyer, W.-D. (2001) DNA repair: CPR for the genome. TIG 17, 546. [Book review of DNA Damage and Repair. Vol. III Advances from Phage to Humans. Eds. J.A. Nikoloff and M.F. Hoekstra. Humana Press, 2001][PDF Loca] Haber, J.E. and Heyer, W.-D. (2001) The fuss about Mus81.[Mini-Review] Cell 107, 551-554. [PubMed] [PDF]

2000: Gos, P., Eicher, B., Kohli, J.and Heyer, W.-D. (2000) No mutagenic or recombinogenic effects of mobile phone fields at 900 MHz detected in the yeast Saccharomyces cerevisiae. Bioelectromagnetics 21, 515-523.[PubMed] [PDF] Schmuckli-Maurer, J. and Heyer, W.-D. (2000) Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae. Chromosoma 109, 86-93. [PubMed] [PDF] Bashkirov, V.I., King, J.S., Bashkirova, E.V., Schmuckli-Maurer, J. and Heyer, W.-D. (2000) DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints. Mol. Cell. Biol. 20, 4393-4404. [PubMed] [PDF] Interthal, H. and Heyer, W.-D. (2000) MUS81 encodes a novel Helix-hairpin-Helix protein involved in the repair of UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol. Gen. Genet. 263, 812-827. [PubMed] [PDF] Mazin, A., Bornarth, C.J., Solinger, J.A., Heyer, W.-D., and Kowalczykowski, S.C. (2000) Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament. Mol. Cell 6, 583-592. [PubMed] [PDF] Boddy, M.N. Lopez-Girona, A., Shanahan, P., Interthal, H., Heyer, W.-D. and Russell, P. (2000) Damage tolerance protein Mus81 associates with FHA1 domain of checkpoint kinase Cds1. Mol. Cell. Biol. 20, 8758-8766. [PubMed] [PDF]

1999: Schmuckli-Maurer, J. and Heyer, W.-D. (1999) The Saccharomyces cerevisiae RAD54 gene is important but not essential for natural homothallic mating-type switching. Molec. Gen. Genet. 260, 551-558. [PubMed] [PDF] Clever, B., Schmuckli-Maurer, J., Sigrist, M., Glassner, B. J. and Heyer, W.-D. (1999) Specific negative effects resulting from elevated levels of the recombinational repair protein Rad54p in Saccharomyces cerevisiae. Yeast 15, 721-740. [PubMed] [PDF] Khasanov, F. K., Savchenko, G. V., Korolev, V. G., Heyer, W.-D. and Bashkirov, V. I. (1999) A new recombinational DNA repair gene from Schizosaccharomyces pombe with homology to E.coli RecA. Genetics 152, 1557-1572. [PubMed] [PDF] Solinger, J. A., Pascolini, D. and Heyer, W.-D. (1999) Active-site mutations in the Xrn1p exoribonuclease of Saccharomyces cerevisiae reveal a specific role in meiosis. Mol. Cell. Biol. 19, 5930-5942. [PubMed] [PDF]

1998: Billinton, N., Barker, M. G., Michel, C. E., Knight, A. W., Heyer, W.-D., Goddard, N. J., Fielden, P. R. and Walmsley, R. M. (1998) Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor. Biosensors & Bioelectronics 13, 831-838. [PubMed] [PDF]

1997: Bashkirov, V. I., Scherthan, H., Solinger, J. A., Buerstedde, J.-M. and Heyer, W.-D. (1997) A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates. J. Cell. Biol. 136, 761-773. [PubMed][PDF] Gos, P., Eicher, B., Siegenthaler, J., Kohli, J. and Heyer, W.-D. (1997) Extremely high frequency electromagnetic fields at low power density do not affect the division of exponential phase Saccharomyces cerevisiae cells. Bioelectromagnetics 18, 142-155. [PubMed] [PDF] Clever, B., Interthal, H., Schmuckli-Maurer, J., King, J., Sigrist, M. and Heyer, W.-D. (1997) Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins. EMBO J. 16, 2535-2544. [PubMed] [PDF] Muris, D. F. R., Vreeken, K., Schmidt, H., Ostermann, K., Clever, B., Lohman, P. H. M. and Pastink, A. (1997) Homologous recombination in the fission yeast Schizosaccharomyces pombe: different requirements for the rhp51+, rhp54+ and rad22+ genes. Curr. Genet. 31, 248-254. [PDF] (B. Clever was a graduate student in my laboratory.) Walmsley, R. M., Billinton, N. and Heyer, W.-D. (1997) Green fluorescent protein as a reporter for the DNA damage-inducable gene RAD54 in Saccharomyces cerevisiae. Yeast 13, 1535-1545. [PubMed] [PDF]

1996: Santos-Rosa, H., Clever, B., Heyer, W.-D. and Aguilera, A. (1996) The yeast HRS1 gene codes for a polyglutamine-rich transcription factor required for initiation of recombination between direct repeats. Genetics 142, 705-716.[PubMed] Kanaar, R., Troelstra, C., Swagemakers, S. M. A., Essers, J., Smit, B., Franssen, J.-H., Pastink, A., Bezzubova, O., Buerstedde, J.-M., Clever, B., Heyer, W.-D. and Hoeijmakers, J. H. J. (1996) Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene: evidence for functional conservation. Curr. Biol. 6, 828-838.[PubMed]

1995: Interthal, H., Bellocq, C., Bähler, J., Bashkirov, V. I., Edelstein, S., and Heyer W.-D. (1995) A role of Sep1(=Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO J. 14, 1057-1066. [PubMed] Heyer, W.-D., Johnson, A. W., Reinhart, U. and Kolodner, R. D. (1995) Regulation and intracellular localization of the Saccharomyces cerevisiae strand exchange protein 1 (Sep1/Xrn1/Kem1), a multi-functional exonuclease. Mol. Cell. Biol. 15, 2728-2736. [PubMed] [PDF] Holler, A., Bashkirov, V. I., Solinger, J. A., Reinhart, U. and Heyer, W.-D. (1995) Use of monoclonal antibodies in the functional characterization of the Saccharomyces cerevisiae Sep1 protein. Eur. J. Biochem. 231, 329-336. [PubMed] Bashkirov, V. I., Solinger, J. A. and Heyer, W.-D. (1995) Identification of functional domains in the Sep1 protein (= Kem1, Xrn1), which is required for transition through meiotic prophase in Saccharomyces cerevisiae. Chromosoma 104, 215-222. [PubMed]

1994: Bähler, J., Hagens, G., Holzinger, G., Scherthan, H. and Heyer, W.-D. (1994) Saccharomyces cerevisiae cells lacking the homologous pairing protein p175SEP1 arrest at pachytene during meiotic prophase. Chromosoma 103, 129-141. [PubMed] Käslin, E. and Heyer, W.-D. (1994) A multi-functional exonuclease from vegetative Schizosaccharomyces pombe cells exhibiting in vitro strand exchange activity. J. Biol. Chem. 269, 14094-14102. [PubMed] Käslin, E. and Heyer, W.-D. (1994) Schizosaccharomyces pombe fatty acid synthase mediates DNA strand exchange in vitro. J. Biol. Chem. 269, 14104-14110. [PubMed] Heyer, W.-D. (1994) The search for the right partner: Homologous pairing and DNA strand exchange proteins in eukaryotes. Experientia 50, 223-233. [PubMed] Heyer, W.-D. and Kohli, J. (1994) Introduction. Experientia 50, 189-191. Homologous recombination (Multi-author review) Edited by Heyer, W.-D. and Kohli, J. Experientia (1994) 50, 189-318. [PubMed]

1993: Bezzubova, O. Y., Schmidt, H., Ostermann, K., Heyer, W.-D. and Buerstedde, J.-M. (1993) Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway through-out the evolution of higher eukaryotes. Nucleic Acids Res. 21, 5945-5949. [PubMed]

Postdoc years: Heyer, W.-D., Evans, D. H. and Kolodner, R. D. (1988) Renaturation of DNA by a Saccharomyces cerevisiae protein that catalyzes homologous pairing and strand exchange. J. Biol. Chem. 263, 15189-15195. [PubMed] Heyer, W.-D. and Kolodner, R. D. (1989) Purification and characterization of a protein from Saccharomyces cerevisiae that binds tightly to single-stranded DNA and stimulates a cognate strand exchange protein. Biochemistry 28, 2856-2862. [PubMed] Heyer, W.-D., Rao, M. R. S., Erdile, L. F., Kelly, T. F. and Kolodner, R. D. (1990) An essential Saccharomyces cerevisiae single-stranded DNA binding protein is homologous to the large subunit of human RP-A. EMBO J. 9, 2321-2329. [PubMed] Erdile, L. F., Heyer, W.-D., Kolodner, R. D. and Kelly, T. F. (1991) Characterization of a cDNA encoding the 70-kDa subunit of human replication protein-A (RP-A), a single-stranded DNA-binding protein involved in DNA replication and recombination. J. Biol. Chem. 266, 12090-12098. [PubMed] Heyer, W.-D., Johnson, A. W., Norris, D. N., Tishkoff, D. and Kolodner, R. D. (1991) Saccharomyces cerevisiae proteins involved in hybrid DNA formation in vitro. Biochimie 73, 269-276. [PubMed] Kolodner, R. D., E. Alani, W.-D. Heyer, A. W. Johnson, D. N. Norris and D. Tishkoff (1992) Saccharomyces cerevisiae proteins that promote hybrid DNA formation in vitro. In: Mechanisms of Eukaryotic DNA Recombination, ed. by M. E. Gottesman and H. J. Vogel, p.127-141. Academic Press. Heyer, W.-D. and Kolodner, R. D. (1993) Enzymology of homologous recombination in Saccharomyces cerevisiae. Progress in Nucleic Acid Research and Molecular Biology 46, 221-271.

Graduate years: Thuriaux, P., Heyer, W.-D. and Strauss, A. (1982) Organization of the complex locus trp1 in the fission yeast Schizosaccharomyces pombe. Curr. Genet. 6, 13-18. Heyer, W.-D., Thuriaux, P., Kohli, J., Ebert, P., Kersten, H., Gehrke, C., Kuo, K.C. and Agris, P.F. (1984) An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the "wobble" base in the anticodon of tRNAs. J. Biol. Chem. 259, 2856-2862. [PubMed] Kohli, J., Munz, P., Aebi, R., Amstutz, H., Gysler, C., Heyer, W.-D., Lehmann, L., Schuchert, P., Szankasi, P., Thuriaux, P., Leupold, U., Bell, J., Gamulin, V., Hottinger, H., Pearson, D. and Söll, D. (1984) Interallelic and intergenic conversion in three serine tRNA genes of Schizosaccharomyces pombe. Cold Spring Harbor Symp. Quant. Biol. 49, 31-40. [PubMed] Amstutz, H., Munz, P., Heyer, W.-D., Leupold, U. and Kohli, J. (1985) Concerted evolution of isoacceptor tRNA genes: intergenic conversion between unlinked serine tRNA genes in Schizosaccharomyces pombe. Cell 40, 879-886. [PubMed] Sipiczki, M., Heyer, W.-D. and Kohli, J. (1985) Preparation and regeneration of protoplasts and spheroplasts for fusion and transformation of Schizosaccharomyces pombe. Curr. Micro. 12, 169-174. Heyer, W.-D., Sipiczki, M. and Kohli, J. (1986) Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element. Mol. Cell. Biol. 6, 80-89. [PubMed] Heyer, W.-D., Munz, P., Amstutz, H., Aebi, R., Gysler, C., Schuchert,P., Szankasi, P., Leupold, U., Kohli, J., Gamulin, V. and Söll, D. (1986) Inactivation of nonsense suppressors in Schizosaccharomyces pombe: Intergenic conversion and mutation hotspots in tRNA genes. J. Mol. Biol. 188, 343-353. [PubMed] Wright, A., Maundrell, K., Heyer, W.-D., Beach, D. and Nurse, P. (1986) Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Plasmid 15, 156-158.[PubMed] Willis, I., Nichols, M., Chisholm, V., Söll, D., Heyer, W.-D., Szankasi, P., Amstutz, H., Munz, P. and Kohli, J. (1986) Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA sequences. Proc. Natl. Acad. Sci. USA 83, 7860-7864. [PubMed] Grossenbacher, A.M., Stadelmann, B., Heyer, W.-D., Thuriaux, P., Kohli, J., Smith, C., Agris, P.F., Kuo, K.C. and Gehrke, C. (1986) Antisuppress-or mutations and sulfur-carrying nucleosides in transfer RNAs of Schizosaccharomyces pombe. J. Biol. Chem. 261, 16351-16355. [PubMed] Szankasi, P., Heyer, W.-D., Schuchert, P. and Kohli, J. (1988) DNA sequence of the ade6 gene of Schizosaccharomyces pombe: Wild-type and mutant alleles including the recombination hot spot allele ade6-M26. J. Mol. Biol. 204, 917-925. [PubMed]

Patents: Walmsley, R. M. and Heyer, W.-D. (1998) Detection of DNA damage 'GFP'. UK Patent 9706414.1