Projects: (1) Rad51-Rad54 interaction during homologous recombination (2) Structure and function of Rad51 and Rad54
Interests: Protein structure, function and protein-protein interaction.
2001 - PhD, Biochemistry, Stockholm University, Sweden 1989 - MSc, Plant Physiology, Lanzhou University, Lanzhou, P. R. China 1983 - BSc, Botany, Lanzhou University, Lanzhou, P. R. China
2007 - Project scientist 2002 - 2007 - Post Doctoral Fellow, Section of Microbiology, UC Davis, USA 2002 - Post Doctoral Fellow, Drexel University College of Medicine, Drexel Univ., USA 1996 - 2001 - PhD student, Dept. of Biochem. & Biophys, Stockholm University, Sweden 1993 -1995 - Visiting Scholar, Hong Kong Baptist University, HK 1983 -1993 - Teacher and Researcher, Dept. of Biology, Lanzhou University, P. R. China
2004 - 2007 Post-doctoral Fellowship, Susan G. Komen Breast Cancer Foundation 1999 - The Best Poster Prize from Bioinformatics '99, the international bioinformatics meeting in Scandinavia, April 15-18, Lund, Sweden
Zhang, X.-P., Galkin, V., Yu, X., Egelman, E. and Heyer, W.-D. (2009) Loop 2 in S. Cerevisiae Rad51 protein regulates filament formation and ATPase activity. Nucleic Acid Res. 37:158-171 [PDF]
Li, X., Zhang, X.-P., Solinger, J., Kiianitsa, K., Yu, X., Egelman, E. and Heyer, W.-D. (2007) Rad51 and Rad54 ATPase activites are both required to modulate Rad51-dsDNA filament dynamics. Nucleic Acid Res. 35(12): 4124-4140. [PubMed] [PDF].
Heyer, W.-D., Li, X., Rolfsmeier, M. and Zhang, X.-P. (2006) Rad54, the Swiss Army knife of homologous recombination? Nucleic Acid Res, 34(15): 4115-4125 .[PubMed] [PDF]
Galkin, V.E., Wu, Y., Zhang, X.-P., Qian, X., He, Y., Yu, X., Heyer, E.-D., Luo, Y. and Egelman, E.H. (2006) The Rad51/RadA N-terminal domain activates Nucleoprotein Filaments. STRUCTURE, 14, 983-992. [PubMed] [PDF] [Cover Illustration] [Preview by Wyman, C. (2006) Monomer Networking Activates Recombinases. Structure 14(6): 949-950 [PDF]]
Zhang, X.-P., Lee, K.-I., Solinger, J.A., Kiianitsa, K., and Heyer, W.-D. (2005) Gly103 in the N-terminal domain of S. cerevisiae Rad51 protein is critical for DNA binding. J. Biol. Chem. 280, 26303-26311.[Pubmed][Pdf file]
Zhang, X.-P., and Glaser, E. (2002) Interaction of chloroplast and mitochondrial signal sequences with organellar hsp70. Trends in Plant Science, 7(1): 14-21 [PubMed] [Pdf file]
Zhang, X.-P., Sjöling, S., Tanudji, M., Somogyi, L., Andreu, D., Eriksson, L. E. G., Gräslund, A., Whelan, J. and Glaser, E. (2001) Mutagenesis and computer modelling approach to study determinants for recognition of signal peptides by the mitochondrial processing peptidase. Plant J., 27 (5):427-438[PubMed] [Pdf file]
Zhang, X.-P., Elofsson, A., Andreu, D. and Glaser, E. (1999) Interaction of mitochondrial presequence with DnaK and mitochondrial hsp70. J. Mol. Biol. 288(1):177-190 [PubMed] [Pdf file]
Pavlov, P. F., Moberg, P., Zhang, X.-P. and Glaser, E. (1999) CNBr chemical cleavage of the overexpressed mitochondrial precursors: a new strategy of construct import competent preproteins. Biochem. J., 341:95-103 [PubMed] [Pdf file]
Full publication list
Photography (see my photos) and website design (see my first design )
Computer hardware:
Intel processors, AMD processors
(1) Heyer lab hikes: Coming soon ... (2) Traveling photos: Coming soon ...
LINKS FOR PROGRAMS / WEBSERVERS
Homology Searching - BLAST Molecular Modeling - ICM, Modeller, Whatif, ECEPPAK Model Verification - Procheck, Whatif, ProsaII, Verify3D, ProQ, ModFOLD, MetaMQAPII, QMEANclust Molecular Docking - Autodock Molecular Graphics - PyMol, Molscript, Raster3D, Grasp, Swiss PdbViewer Residue Conservation - Sequence logo Sequence Alignment - ClustalX, CLustalW, Bioedit, EMBOSS, eBioX (Mac) and eBioTools (Mac) Alignment Editing - Bioedit, Gendoc
MOLECULAR BIOLOGY
DNA double digest table Restriction Enzyme Database NEBcutter Protein interaction Protease inhibitors
QUESTIONS AND ANSWERS
1. How to visualize sequence files on PC, Mac and Linux: PC users: Chromas Lite(Pro), SequenceScanner, Bioedit, Staden, EMBOSS, VectorNTI. Mac users: 4peaks, Staden, EMBOSS, ?? Linux users: EMBOSS, Staden, or the PC/Windows programs through Wine, or VMware.
2. How to run Windows and Linux (Unix) applications on the same computer? (1)Dual/multi-operating systems may be the best way if you don't need to talk between Windows application and Linux application. (2)If you do and mainly depend on Windows, Cygwin may help. (3) Wine and VMware may be the good solution for Linux (Unix) users. Based on my experience, VMware is extremely slow.
3. How to ...
Links for Linux fans Phoronix
Databases - NCBI, Swiss-Prot, PDB, Yeast Genome Database, Human Pathway Interactions, MIPS, Protein interaction (based on structure), Protein interaction (Genetic and Physical interaction), Finley Lab Interactions Databases, BioGRID,DIP
Protein lounge (Commercial databases)
Concensus prediction for protein secondary structure - NPSA
Full length protein 3D structural prediction servers - I-TASSER at the University of Kansas - Robetta Server at the University of Washington
Concensus fold recognition servers - Meta server from bioinfo Bank, Poland - Pcons - MetaTASSER at the Georgia Institute of Technology - LOMETS at the University of Kansas
Other homology template searching servers - HHpred in Germany - RAPTOR Server at the University of Chicago - SP3 fold recognition - Inub at the University at Baffalo
On-line molecular docking server - GRAMM-X
Signal peptide prediction and protein localization - MitoProt, SignalP (V3.0), SignalP (V2.0b2), TargetP, ChloroP, Predotar, ProtComP, NNPSL, PSORT, Yeast protein localization, PredictNLS
Phosphorylation site prediction - NetPhos, PhosphoBase
Links for DNA repair DNA Repair Wiki DNA Repair Interest Group at NIH DNA Damage Response and Repair Mechanisms DNA Repair at ELSEVIER DNA Repair by John W. Kimball Mutation, Mutagens, and DNA Repair by Beth A. Montelone Human DNA Repair Genes by Cancer Research UK The book of "DNA Repair and Mutagenesis" DNA Repair from WormBook DNA Repair from Reactome
Other info:
memberships
2002 - Present: American Society for Biochemistry and Molecular Biology 2003 - 2006: Internation Computational Biological Society
Updated on Jan. 09, 2009
